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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
27/06/2023 |
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Actualizado : |
28/06/2023 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
DE TRAVENSOLO, R.F.; FERREIRA, V.G.; FEDERICI, M.; DE LEMOS, E.G.M.; CARRILHO, E. |
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Afiliación : |
REGIANE FÁTIMA DE TRAVENSOLO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; VINÍCIUS GUIMARÃES FERREIRA, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELIANA GERTRUDES MACEDO DE LEMOS, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, Jaboticabal, SP, Brazil; EMANUEL CARRILHO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil. |
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Título : |
Microarrays application in life sciences: The beginning of the revolution. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
In: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25 |
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ISBN : |
978-303082380-1; eISBN: 978-303082381-8 |
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DOI : |
10.1007/978-3-030-82381-8_25 |
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Idioma : |
Inglés |
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Notas : |
Correspondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br -- |
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Contenido : |
Previous to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd. |
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Palabras claves : |
DNA analysis; Microarray applications; Microarrays. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 01965naa a2200229 a 4500
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024 7 $a10.1007/978-3-030-82381-8_25$2DOI
100 1 $aDE TRAVENSOLO, R.F.
245 $aMicroarrays application in life sciences$bThe beginning of the revolution.$h[electronic resource]
260 $c2022
500 $aCorrespondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br --
520 $aPrevious to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd.
653 $aDNA analysis
653 $aMicroarray applications
653 $aMicroarrays
700 1 $aFERREIRA, V.G.
700 1 $aFEDERICI, M.
700 1 $aDE LEMOS, E.G.M.
700 1 $aCARRILHO, E.
773 $tIn: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
22/02/2014 |
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Autor : |
Verges, R. ; Diaz de Ackermann, M. ; Troche, L. ; Tavella, C.M. |
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Título : |
Mejoramiento genetico de trigo duro |
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Fecha de publicación : |
1993 |
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Fuente / Imprenta : |
ln: Dia de Campo Cultivos de Invierno Young, 1993 nov 5 : Young [Memorias]. Montevideo (Uruguay): INIA, 1993. |
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Páginas : |
p20-22 |
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Serie : |
Dia de Campo Cultivos de Invierno (INIA) |
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Idioma : |
Español |
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Thesagro : |
ENSAYOS DE VARIEDADES; TRITICUM DURUM. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 00592naa a2200193 a 4500
001 1046239
005 2014-02-22
008 1993 bl uuuu u00u1 u #d
100 1 $aVERGES, R.
245 $aMejoramiento genetico de trigo duro
260 $c1993
300 $ap20-22
490 $aDia de Campo Cultivos de Invierno (INIA)
650 $aENSAYOS DE VARIEDADES
650 $aTRITICUM DURUM
700 1 $aDIAZ DE ACKERMANN, M.
700 1 $aTROCHE, L.
700 1 $aTAVELLA, C.M.
773 $tln: Dia de Campo Cultivos de Invierno Young, 1993 nov 5 : Young [Memorias]. Montevideo (Uruguay): INIA, 1993.
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INIA La Estanzuela (LE) |
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